سعیده دهقانپور فراشاه - Former graduate student of Plant Pathology College of Agriculture, Isfahan Univ. of Technology, Isfahan, Iran
آقا فخر میرلوحی - Associate Prof. of Plant Breeding, College of Agriculture, Isfahan Univ. of Technology, Isfahan, Iran (E-mail: mirlohi@cc.iut.ac.ir)
بهرام شریف نبی - Assist. Prof. of Mycology College of Agriculture, Isfahan Univ. of Technology, Isfahan, Iran (E-mail: sharifna@cc.iut.ac.ir),

Endophytic fungi have mutualistic relationship with the plant family Poaceae. These fungi confer characteristics such as yield increase and biotic and abiotic stress resistance to host plants. Endophytes are classified in the familyClavicipitaceae. The endophytes spend all their life cycle in the aerial parts of plant hosts and live intercellularly. In the present investigation, endophytic fungi were isolated from seed and leaf sheath of Festuca arundinaceae, F. ovina, F. pratensis, Bromus tomentellus, Melica persica and Lolium prenne. Genomic DNA was extracted and three sets of primers: ITS۱/ITS۴, IS۱/IS۳ and ۱۱۱/۱۱۲ were used to detect and identify endophytes. The results of PCR with three paires of primers indicated that most of isolates used in research were endophytic fungi belonging to Neotyphodium and isolates of F. arundinacea were N. coenophialum.

Endophytic Fungi, Neotyphodium, PCR-RFLP, Taxonomy