The effect of gassericin A on the gene expression of tumor Necrosis Factor alpha (TNF-α) in differentiated preadipocyte 3T3-L1 cells

Introduction & Objectivesc: clinical studies report a growing increase in the mortality caused by obesity and obesity-related diseases. There is a close link between obesity, adipocyte inflammation and colon cancer, that may threaten health. Factors such as nutrition, genetic background, and activity may affect adipocyte metabolism. One of the other factors which has been recently studied is intestinal microbiota. Microbiota refers to commensal microbial population in the body. The highest population of microbiota has been observed within the colon. These microorganisms produce different compounds such as short chain fatty acids, lipopolysaccharides and bacteriocins. Bacteriocins are peptide composed of different amino acids, which are released to control the other microorganisms within the intestine. One of these peptides is Gassericin A, which has a cyclic structure containing 58 amino acids. This bacteriocin is produced by the intestinal Lactobacillus gasseri LA39. According to the present databases, there is no report regarding the effect of this bacterial product on metabolic processes.Materials & Methods: In this study, L gasseri was cultured in Dob MRS broth media. Gassericin A was extracted using column chromatography (HIC/RP-8). Thereafter, the peptide was verified according to its specific antibacterial effect on L delbrueckii bulgaricus and also employing tricine-SDS-PAGE method. To investigating the possible effects of gassericin A on the expression of the TNF-α gene, the preadipocyte 3T3-L1 cells were first allowed to differentiate into adipocytes using IBMX, dexamethasone, and insulin. The gene expression of TNF-α was assessed using quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay.Results: Gassericin A cause a significant reduction in the expression of TNF-α gene in adipocytes.Conclusion: This study suggests that gassericin A produced by Lactobacillus gasseri LA39 prevents inflammation in adipocytes via downregulation of TNF-α. This process may play role in prevention of obesity and cancer.

کلیدواژه ها:

microbiota ، Lactobacillus gasseri ، gasseircin A ، TNF-α ، 3T3-L1 cell