Anti-biofilm effect of crude bacteriocin derived from Lactobacillus brevis DF۰۱ on Escherichia coli and Salmonella Typhimurium

Background and Aim : Biofilm is a community of microorganisms that adhere to abiotic or biotic surfaces, and is problematic in a wide range of food industries, as well as to human health. Although bacteriocin from lactic acid bacteria is well known as a natural antimicrobial agent in food preservation, it has been poorly investigated that bacteriocin inhibits the biofilm formation of foodborne pathogens. In this study, we demonstrated that bacteriocin produced by Lactobacillus brevis DF۰۱ was partially purified and investigated whether the bacteriocin inhibited the biofilm formation of Escherichia coli and Salmonella Typhimurium. Assessment by the microtiter plate method, as well as by fluorescence and scanning electron microscopy, showed that the biofilm formation of E. coli and S. Typhimurium was reduced when the bacteria was co-incubated with crude bacteriocin of L. brevis DF۰۱ (DF۰۱ bacteriocin). Although pre-treated DF۰۱ bacteriocin also significantly inhibited the biofilm formation of E. coli and S. Typhimurium (P=۰.۰۰۳۵ and P=۰.۰۰۰۳, respectively) post-treatment with DF۰۱ bacteriocin did not significantly inhibit the biofilm formation (P=۰.۱۳۱۴ for E. coli and P=۰.۲۹۳۹ for S. Typhimurium), suggesting that DF۰۱ bacteriocin interfered with biofilm formation, but did not disrupt the established biofilm of E. coli and S. Typhimurium. In addition, biofilms of both E. coli and S. Typhimuriun on the surface of stainless steel coupons were decreased in the presence of DF۰۱ bacteriocin. Taken together, these results suggest that DF۰۱ bacteriocin may be applied to control the biofilm formation of E. coli

کلیدواژه ها:

Bacteriocin ، Lactobacillus brevis ، Biofilm ، Antimicrobial activity ، Foodborne pathogens