Insecticide-enzyme interaction: spirotetramate with alpha amylase in the Agonoscena pistaciae

Spirotetramat is a new insecticide that recommended to control of pistachio psylla, onion thrips and soft scales in Iran. This chemical is lipid biosynthesis inhibitor that acts with blocking of aceyl Coenzyme A carboxylase. In this study, an effect of spirotetramte on the alpha amylase activity was studied. The final concentrations of insecticides were ۰ (control), ۱۰۰, ۲۰۰, ۴۰۰, ۸۰۰ and ۱۶۰۰ ppm. Adult insect were collected about of Birjand and two adults transfer to one ml phosphate buffer (pH:۶.۵). Samples were homogenized and after centrifuging (۱۵۰۰۰ rpm, -۴ centigerade, ۱۵ min), supernatant kept in in the -۱۸ centigrade. Fifty microliters of toxic treatment incubated with ۱۰۰ enzyme solution foe ۳۰ min in the room temperatures. Enzyme activity was measurement using auto analyzer and alpha amylase and total protein kits. Results showed that there is no significant difference between control and ۱۰۰, ۲۰۰ ppm. Also, the significant reduction was occurred in the ۴۰۰ and ۸۰۰ ppm, with ۵۵ and ۸۴ percent in comparison to the control. The enzyme activity was removed completely in the highest toxic concentration treatment. Result suggests that alpha amylase as key enzyme in the carbohydrate metabolism can be reported as new target of mode of action for spirotetramt.

کلیدواژه ها:

Autoanalyzer ، Digestive system ، Homoptera ، Kit ، Inhibitor