Differentiation of Adipose-derived Stem Cells into Schwann Cell Phenotype in Comparison with Bone Marrow Stem Cells
محل انتشار: مجله علوم پایه پزشکی ایران، دوره: 13، شماره: 3
سال انتشار: 1389
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 177
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شناسه ملی سند علمی:
JR_IJBMS-13-3_003
تاریخ نمایه سازی: 3 آبان 1400
چکیده مقاله:
Objective(s) Bone marrow is the traditional source of human multipotent mesenchymal stem cells (MSCs), but adipose tissue appears to be an alternative and more readily available source. In this study, rat adipose-derived stem cells (ADSCs) were induced to differentiate into Schwann-like cells and compared with rat bone marrow stem cells (BMSCs) for their Schwann-like cells differentiation potential. Materials and Methods BMSCs and ADSCs were characterized for expression of MSCs-specific markers, osteogenic and adipogenic differentiation. They were induced to differentiate into Schwann-like cells and analyzed for expression of the Schwann specific markers. The immunocytochemical differentiation markers were S-۱۰۰ and real time quantitative Real-time polymerase chain reaction (RT-PCR) markers were S۱۰۰, P۷۵ and glial fibrillary acidic protein (GFAP). ۳-(۴, ۵-Dimethylthiazol- ۲-yl)-۲, ۵-diphenyltetrazolium bromide (MTT) assay and Annexin V-Fluorescein isothiocyanate (FITC)/ Propidium iodide (PI) double labeling method were employed to detect early stage cell apoptosis. Results BMSCs and ADSCs showed similarities in expression of the MSC-specific markers, osteogenic and adipogenic differentiation. Both quantitative RT-PCR and immunocytochemical analysis demonstrated that BMSCs and ADSCs had equal expression of the Schwann-specific markers following Schwann-like cells differentiation. However, gene expression of P۷۵ was higher in BMSCs compared with ADSCs. MTT assay and flow cytometry found that of the total BMSCs and ADSCs in the culture medium, ۲۰% to ۳۰% of the cells died, but the remaining cell population remained strongly attached to the substrate and differentiated. Conclusion Comparative analysis showed that Schwann-like cell differentiation potential of ADSCs was slightly decreased in comparison with BMSCs. Therefore, BMSCs are more favorable choice than ADSCs for tissue engineering.
کلیدواژه ها:
نویسندگان
Zolikha Golipoor
School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Iraj Ragerdi Kashani
School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Mohammad Akbari
School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Gholamreza Hassanzadeh
School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Fatemeh Malek
School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Reza Mahmoudi
School of Medicine, Yasouj University of Medical Sciences, Yasouj, Iran
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