Genetic Variations of Tumor Necrosis Factor –α-۳۰۸ and Lymphtoxin-α+۲۵۲ in Non-Hodgkin Lymphoma and Acute Lymphoblastic Leukemia Patients

  Objective(s): Non- Hodgkin lymphoma (NHL) and acute lymphoblastic leukemia (ALL) are two main hematological malignances which have been driven from lymphoid tissue. Genetic polymorphisms in tumor necrosis factor-α (TNF-α) -۳۰۸ and lymphotoxin-α (LT-α) +۲۵۲ may affect their transcription and expression which leads to their high plasma level. The frequency of the TNF-α (-۳۰۸) and LT-α (+ ۲۵۲) polymorphisms are different for NHL and ALL cases in various populations with different ethnicity. This research is designed to investigate the prevalence and association of TNF-α (-۳۰۸) and LT-α (+ ۲۵۲) polymorphisms from NHL and ALL in Azarian patients and healthy individuals from Northwestern part of Iran.   Materials and Methods: Seventy subjects with ALL and ۶۸ NHL, along with another ۱۳۰ healthy subjects as control group took part in this study. Genomic DNA was extracted, then genetic polymorphisms in TNF-α and LT-α genes were analyzed with the PCR-RFLP and NCOI as restriction enzyme. A statistical analysis was performed by chi-square test using SPSS software. A P-value of <۰.۰۵ was considered statistically significant. Results: A statistically significant difference of LT-α polymorphism was in NHL patients and control (P-value= ۰.۰۰۸) but there was not any association of TNF-α polymorphism between NHL patients and control group. A significant association for TNF-a variant was in ALL and control (P-value =۰.۰۰۵), however, there was no relationship about LT variant between ALL and control. Conclusion: The results show that there are significant differences between TNF-α (-۳۰۸) and LT-α (+۲۵۲) genetic polymorphisms respectively in ALL and NHL patients with control group from Northwestern part of Iran.