BTG۱ could be used as a potential marker of biological diagnosis of gastric cancer: Dezful University of Medical Sciences (DUMS)

Introduction: gastric cancer is the third leading cause of cancer death in the world. Timely diagnosis and treatment is one of the most effective factors in improving and treating this disease, and in this regard, an effective screening method is needed. Because the B۱ cell transfer gene (BTG۱) is a highly regulated gene that can regulate cell proliferation and differentiation, it can be considered as a possible biomarker for GC diagnosis. Methods: A total of ۶۰ samples of GC, atrophy and healthy control were collected based on inclusion and exclusion criteria. Trizol solution kit was used to extract RNA from the obtained samples. RNA samples were analyzed for qRT-PCR using specific primers designed against BTG۱ and GAPDH genes. Finally, qRT-PCR results were analyzed by ΔCt -۲ method and various statistical tests using SPSS software. Results: We analyzed the expression and prognosis of BTG۱ based on TCGA and found that low expression of BTG۱ was associated with the prognosis of GC. qRT-PCR showed that BTG۱ expression was lower in gastric cancer and atrophic tissue than in control tissue. Pathological analysis showed that BTG۱ plays an important role in regulating growth, tumor formation, apoptosis and cell cycle. Western blot showed that BTG۱ inhibits gastric cancer. Conclusion: It can be concluded that decreased BTG۱ expression can act as an accurate biomarker for GC. This gene can also be an indicator of GC pathogenicity. These results could indicate possible diagnostic and therapeutic applications of BTG۱ for GC.

کلیدواژه ها:

BTG۱ ، Gastric cancer ، atrophic