Silibinin inhibits MCF۷ breast cancer cell proliferation and migration by GREB۱ targeting

Backgrounds: Growth Regulation by Estrogen in Breast Cancer ۱ (GREB۱) is an estrogenreceptor (ER) target gene and its expression is correlated with estrogen levels in breast cancer.ER-positive breast cancer cell lines, express GREB۱ at high levels. GREB۱ helps transcriptionregulation of ER target genes. Silibinin is a bioactive component of Silymarin from seeds of milkthistle. This component possesses several pharmacological properties such as anti-inflammatory,antioxidant, anti-cancer, and estrogenic effects. Therefore, the aim of the present study was theassessment of Silibinin on proliferation, migration, and GREB۱ expression.Materials and Methods: MCF۷ breast cancer cells cultured in RPMI۱۶۴۰ comprising FBS(۱۰%), ۱۰۰ U/ml penicillin/ streptomycin at ۳۷°C in a humidified atmosphere containing ۵%CO۲. Cells were treated with different concentrations of Silibinin and then cell cytotoxicity wasinvestigated by the MTT assay. Scratch test used to evaluate the rate of cell migration. Todetermine the expression of the GREB۱ gene, qPCR in Silibinin treatment was analyzed.Results: MTT analysis showed that in MCF۷ cells treated by Silibinin, cell proliferation issignificantly inhibited. Furthermore, the % of an open wound in migration analysis in bothcontrol and treated cells indicates that Silibinin prevented cell migration. Gene expressionanalysis revealed downregulation of the GREB۱ gene in MCF۷ cancer cells.Conclusion: Silibinin treatment has an inhibitory effect on MCF۷ breast cancer cell proliferationand migration, also downregulates the GREB۱ gene. Therefore, the GREB۱ targeting bySilibinin in the MCF۷ cell line can be proposed as a therapeutic pathway in breast cancer.

کلیدواژه ها:

Silibinin ، Anticancer ، MCF۷ ، GREB۱