استفاده از دکستروز مونوهیدرات جهت القای هایپرگلایسمی در ماهی مدل زبرا (Danio rerio)

The aim of the present study was to establish hyperglycemia in zebrafish (Danio rerio) using dextrose monohydrate as a suitable and inexpensive alternative to glucose in the diabetic process. A number of ۹۰ adult fish with an average weight of ۳۱۶.۳۸ ± ۵۳.۷۴ mg were selected and after adaptation were subjected to different doses of dextrose monohydrate for ۲۴ days in two treatments of diabetic and control treatments each with three replicates. Blood samples were taken at the beginning of the experiment and also on the seventh, sixteenth and twenty-fifth days after inducing hyperglycemia to check the glucose levels. Comparison of blood glucose levels between the diabetic and control treatments showed an increase in fasting blood glucose levels from ۴۱.۱۷ to ۱۸۸.۶۷ after immersion in dextrose solution for ۲۴ days. Also in glucose tolerance test in diabetic treatment, glucose level remained above ۶۰۰ mg/dl after ۹۰ minutes. In general, the results showed that the use of dextrose in a gradual manner causes hyperglycemia and this method can be used to induce type ۲ diabetes.

کلیدواژه ها:

دیابت ، دکستروز ، گلوکز ، ماهی زبرا