Anti-cancer effect of Mandragora.autumnalisextracts on the Hela cell line.

Background: It has been shown that plant-derived agents arebeing used for cancer treatment.In this study, various extracts of Mandragora autumnalis weretested for cytotoxic activity on Hela (Human cervical carcinoma)cell line using the MTT assay.We conducted this study to evaluate the in vitro cytotoxic activityof medicinal plant extracts that we collected from Khuzestan,southwest of Iran.Materials and Methods: Ethanolic and methanolic extractswere prepared from leaf and root of M.autumnalis. Hela cellswere treated with leaf and root samples of M.autumnalis at differentconcentrations (۰,۰.۲۵,۰.۵, and ۱ mg/ml) for ۴۸ hours.cell proliferation was measured by MTT assay.Results: Results of the performed assay showed that methanolicextract of M.autumnalis root had the highest anti proliferativeactivity compared to other extracts in the Hela cell line.This research also demonstrated that as the concentration andduration of treatment increased, the viability of the cells decreasedsignificantly compared to the control samples.Conclusion: Methanolic extract of M.autumnalis root couldbe considered as potential source of anticancer compounds butfurther studies are necessary for isolation and identification ofbiologically active substances.However, we concluded that all extracts from this plant showedIC۵۰ in potential range for further investigation on other activitiesagainst cancer cells.Considering that M.autumnalis is capable of inducing apoptosisand decreasing cell activity, it appears to be a suitable option forinhibiting the growth of tumor cells.

کلیدواژه ها:

Mandragora autumnalis ، Cancer ، Cytotoxicity ، MTTassay