Differential Expression of MiR-۱۸b in PBMC from T۱D Patients in Isfahan population

Background: Type ۱ diabetes (T۱D) is caused by cell-mediated autoimmune attack on pancreatic beta-cells. Previous studies highlight the role of microRNAs (miRNAs) in the pathogenesis of T۱D. MiRNAs are small non-coding RNAs involved in the regulation of gene expression post-transcriptionally. In this work, miR-۱۸b was chosen and the differential expression of it was measured between T۱D patients and healthy controls from Isfahan population. Materials and Methods: MiR-۱۸b was selected using Bioinformatics studies by miRWalk software. ۲۲ T۱D patients and ۱۸ healthy controls from Isfahan population were enrolled in this study. Total RNA of the peripheral blood mononuclear cells (PBMCs) samples were extracted. After cDNA synthesis, the expression profile of miR-۱۸b quantified by means of qPCR method in patients and controls. Finally the results were statistically analyzed. Results: In this study despite our hypothesis, the expression levels of miR-۱۸b didn’t show any significant difference between T۱D patients and healthy controls (p value: ۰.۱۴۵). Conclusion: Due to the results of our experimental analysis, it seems that miR-۱۸b doesn’t have any association with T۱D disease in Isfahan population.

کلیدواژه ها:

type ۱ diabetes (T۱D) ، microRNA ، miR-۱۸b ، qPCR ، miRWalk