Modification of clay with collagen in silk-clay composite biomaterial for bonetissue engineering

The purpose of our investigation is the formation of a novel composite scaffold by modificationof montmorillonite (MMT) with collagen in silk scaffold. Silk fibroin serves as a promising basematerial suitable for bone tissue applications. Montmorillonite is a source of osteoinductivespecies which used as bone repair biomaterial systems. On the other hand, using collagen wereimproved cell adherence and proliferation. In this project different MMT particle–silk (SM)composite biomaterial scaffolds were compared with silk/MMT/collagen (SMC) scaffolds. Whencollagen was added to MMT solution at physiological pH, modification of clay occurred becauseof the interaction between MMT and collagen which scaffolds was characterized using Fouriertransform infrared spectroscopy (FT-IR), x-ray diffraction (XRD) and scanning electronmicroscopy (SEM) techniques. The biocompatibility of scaffolds was evaluated by MTT assayusing rabbit osteoblast cells. Also good swelling and porosity characters of the compositescaffold caused to its improved mechanical properties. By using this method; collagen isinteracted with the MMT solution at room temperature at physiological pH and therefore it is notdenatured and remain stable during the duration of the experiments, a faster apatite precipitationon the scaffolds and the biomaterials supported the attachment and proliferation of rabbitosteoblast cells, maintaining high cell viability.

کلیدواژه ها:

Silk ، montmorillonite ، biomineralization ، freeze dry ، collagen