A metabonomic study on samples of cutaneous leishmaniasis and correlation with the level of selenium of their blood serum

Metabonomics is formally defined as ‘the quantitative measurement of the multi-parametric metabolic response of living systems to path physiological stimuli or genetic modification. Multivariate statistical methods provide an expert means of analyzing and maximising information recovery from complex NMR spectral data. Detailed inspection of NMR spectra and integration of individual peaks can give valuable information on dominant biochemical changes; samples are serum of human blood that divided into two group1.normal samples 2.patient samples (cutaneous leishmania). Leishmania is a parasite that affects vertebrates with the disease called Leishmaniasis. It has a complex life cycle. When an infected Phlebotomid sand fly (Phlebotomus) takes a blood meal it infects the vertebrate host with promastigote metacyclic forms. Within a short time the promastigotes are taken up by macrophages, the first line of defence of the immune system. The promastigote then loses its flagella and transforms into the amastigote form. Once internalized in a phagosome the macrophage lysosome fuses with the phagosome to from a phagolysosome containing the parasite. NMR method is CPMG (Carr–Purcell–Meiboom–Gill) with 200 number scans. The CPMG experiment, like the simple spin-echo experiment, provides useful spectral simplification because broad resonances from macromolecules and membrane bi-layers are attenuated, thus giving improved resolution of resonances from low molecular weight (Fig 1).