The effects of free and magnetic nanostructure-bound deep eutectic solvent on structure and function of firefly luciferase

In this study, we applied free and magnetic nanostructure-bound choline based deep eutectic solvent (cholinechloride/glycerol at 1:2 molar ratio) as inexpensive, non-toxic, biodegradable solvents for the evaluation of their effectson structure and function of firefly luciferase from Lampyris turkestanicus. Magnetic nanostructure-bound deep eutecticsolvent (DES) have been synthesized by covalent bonding of DES-silane on magnetic silica nanostructures. So, the effectsof [γ-fe2o3@SiO2][ChCl/glycerol] were investigated on structure and function of luciferase using bioluminescence assay.Finally, we observed that, in the presence of free and nanostructure-bound DES in the reaction medium enzymatic kineticparameters such as activity, thermostability, and remaining activity will be improved. In addition, this solvent, in its freeand nanostructure-bound forms, has no considerable effect on Km value of luciferase for its both substrates (luciferineand adenosine-5´-triphosphate).