Molecular typing of Clostridium perfringens isolated from minced meat
سال انتشار: 1394
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 320
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شناسه ملی سند علمی:
JR_IJVST-7-1_004
تاریخ نمایه سازی: 11 تیر 1396
چکیده مقاله:
The aim of this study was to determine the presence of toxin genes in Clostridium perfringens isolated from 200minced meat samples using molecular typing. For this purpose, after isolation of Clostridium perfringens using conventional culture method and confirmation by specific 16Srd NA gene PCR, alpha (cpa), beta (cpb), beta 2 (cpb2),epsilon (etx), iota (iA) and enterotoxin (cpe) genes were investigated by multiplex PCR.Out of 200 minced meat samples, 25 (12.5%) were determined as contaminated with C. perfringens and m-PCR results demonstrated that, out of these 25 isolates, 18 (81 %) possessed only the cpa gene (type A), while 4 (18 %) carried the cpa and cpb2 toxin genes (type A-cpb2+). Furthermore, the results showed1 isolate (4 %) as type B, 1 isolate (4 %) as type D and 1 isolate (4 %) as type E. This study reports the first cpb2 positive type B of C. perfringens in minced meat. Our results also indicate that C. perfringens type A is the most common type in minced meat
کلیدواژه ها:
نویسندگان
Asma Afshari
Ph.D Student of Food Hygiene, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Abdollah Jamshidi
Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Jamshid Razmyar
Department of Clinical Science, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Mehrnaz Rad
Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran