Solid-Phase Extraction of Metoclopramide from Biological Fluids and Human Urine By Means Of Molecularly Imprinted Polymers

In this work, water-compatible molecularly imprinted polymers (MIPs) were prepared for selective solid-phase extraction of metoclopramide (MCP) from complex matrices such as biological fluids and human urine followed by its UV spectroscopic determination at 309 nm. The effective factors influencing the precipitation polymerization have been studied. Molecular recognition properties, binding capability and selectivity of the MIPs were evaluated and the results revealed the obtained MIPs have high affinity for MCP in aqueous environment. Equilibrium binding experiments were done to assess the performance of the MIP relative to non imprinted polymer (NIP). After optimization of SPE procedure, successful imprinting was confirmed by comparison of the recoveries between NIP (10%) and MIP (95.5%) particles. The binding capacity of the MIP for MCP was determined to be 113 mg g-1 (319 μmol g-1) accuracy and precision was checked by the liquid chromatographic technique and the results did not present significant difference at 95% confidence levels according to the t test.