The role of lactate dehydrogenase in the progression of breast cancer cell lines MCF-7 & MDA-MB231 and its inhibition by oxamate
محل انتشار: سیزدهمین کنگره بین المللی سرطان پستان
سال انتشار: 1396
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 575
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شناسه ملی سند علمی:
ICBCMED13_129
تاریخ نمایه سازی: 2 تیر 1397
چکیده مقاله:
Introduction & Aim: Higher rate of glycolysis has been long observed in cancer cells, as a vital enzyme in glycolysis, lactate dehydrogenase (LDH) has been shown with great potential as an anti-cancer target. Here, we investigated the effects of oxamate, one classic inhibitor of LDH-A in MCF-7 & MDA-MB231 cell lines.Methods: In this study, after culturing cancer cells and adding oxamate to cells, the proliferation of cells and the activity of the enzyme lactate dehydrogenase as the main marker of glycolysis pathway was determined by MTT assay and LDH activity assay kit respectively.Results : The results showed that oxamate significantly suppressed the proliferation of MCF-7 & MDA-MB231 cells. Furthermore, in the present study, we found that the power of oxamate as an inhibitor of LDH-A in MDA-MB231 greater than to MCF-7.Conclusion: Collectively, these results suggest that LDH-A may serve as a promising therapeutic target for breast cancer treatment.
کلیدواژه ها:
نویسندگان
Hadi Kalantar
Department of Toxicology and Pharmacology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Taraneh Moeini Zanjani
Department of Pharmacology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Masoumeh Sabet Kasaei
Department of Pharmacology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Ali Shahriari
Department of Biochemistry, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran.