ANTIBIOTIC RESISTANCE PATTERN AND FREQUENCY OF EXTENDED SPECTRUM Β-LACTAMASES (ESBLS) IN GRAM NEGATIVE UROPATHOGENIC BACTERIA ISOLATED FROM URINE SAMPLES IN BANDAR TORKAMAN, IRAN

Background and Aim:Antibiotic resistance is rising in Gram negative bacteria causing urinary tract infections (UTI). The aim of this study was to assess antibiotic resistance pattern and frequency of extended spectrum β-lactamases (ESBLs) in Gram negative uropathogenic bacteria isolated from urine samples referred to clinical laboratories of Bandar Torkaman, Iran.Methods:In this study from March to May 2017, midstream urine samples were collected from patients who had clinical manifestation of UTI. In positive samples uropathogenic bacteria were identified by biochemical tests and their antibiotic resistance pattern was determined by disk diffusion method. Then, phenotypic confirmatory test was performed for detection of ESBLs producersResults:Out of 1388 urine samples, 96 samples were positive for urinary infection. The most commonly isolated were as follows: Escherichia coli (85), Klebsiella pneumonia (5), Enterobacter cloace (4) and pseudomonas aerouginosa (2). The highest resistance rates among the majority of the isolates were observed for ampicilin and nalidixic acid. The lowest resistance of E. coli isolates was observed to Norfloxacin. Out of 35 cefotaxime resistant isolates, 29 isolates were positive for ESBLs.Conclusion:In this study, the most prevalent cause of UTI was E. coli (88.5%). The frequency of ESBLs in E. coli isolates was similar to the results of other studies in this field but it was very high in K. pneumonia. The results of this study demonstrated increasing trend of resistance in extended-Spectrum cephalosporins. Therefore before prescribing antibiotics by physicians performing an accurate Antibiogram test seems rational and is an inevitable necessity.

کلیدواژه ها:

Antibiotic ، ESBLs ، Gram Negative ، Frequency ، Uropathogen